HPLC Determination of Famotidine in Tablets on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Famotidine
F amotidine is an over-the-counter histamine H2-receptor antagonist which is often used to decrease the amount of acid in the stomach. It works through blocking histamine-2 receptors in the stomach lining.
Famotidine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 266 nm |