HPLC Determination of Dursban on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpyrifos-methyl (Dursban) .
Dursban is a highly toxic organophosphate with the chemical formula C9H11Cl3NO3PS . It works through interrupting the electrochemical processes in nerves. This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.
Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Formate buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 280 nm |