Determination N-Acetyl-L-Cysteine on Primesep SB Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of N-Acetyl-L-cysteine .
N-Acetyl-L-Cysteine ( NAC ), also known as Acetylcysteine , is the N-acetyl derivative of the naturally occurring amino acid L-cysteine with the chemical formula C5H9NO3S . It is primarily used to treat paracetamol overdoses, chronci bronchopulmonary disorders, and lactobezoar.
N-Acetyl-L-cysteine can be retained on Primesep SB , a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 200 nm.
Methodenparameter
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Na2HPO4pH 4.0 – 50 mM |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 200 nm |