Determination Pantoprazole on Obelisc R Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole , Pantoprazole sodium .
Pantoprazole is a proton pump inhibitor (PPI) with C16H15F2N3O4Smolecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.
Pantoprazole , Pantoprazole sodium can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Pantoprazole can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV at 290 nm, ELSD, CAD or LC/MS.
Methodenparameter
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN -35% |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 290 nm |