Determination Omeprazole on Obelisc R Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Omeprazole .
Omeprazole is a proton pump inhibitor (PPI) with the chemical formula C17H19N3O3S . It is a medication that decreases the amount of acid produced in the stomach. Omeprazole has a bactericidal effect on Helicobacter pylori.
Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties. Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer. Can also be UV detected at 300 nm.
Methodenparameter
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN -20-50% in 15 min + 5 min hold |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 300 nm |