HPLC Determination of Tebuconazole on Newcrom B
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tebuconazole .
Tebuconazole is a triazole fungicide with the chemical formula C16H22ClN3O . It is used against to plant pathogenic fungi. Despite the U.S. Food and Drug Administration considering this fungicide safe, it is considered a possible carcinogen with the rating of C. According to the World Health Organization, it has moderate toxicity and is slightly hazardous.
Tebuconazole can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Gradient AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 275 nm |