HPLC Determination of Amlodipine in Norvasc Tablets
High Performance Liquid Chromatography (HPLC) Method for Analysis of Amlodipine besylate
Amlodipine is a prescription typically sold under the brand name “Norvasc.” It is used to treat high blood pressure and artery disease. It works by preventing calcium from entering cells through blocking L-type calcium channels.
Amlodipine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 238 nm |