HPLC Determination of Didodecyldimethylammonium Bromide on Primesep SB Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Didodecyldimethylammonium bromide , Bromide
Didodecyldimethylammonium Bromide is a double-chain cationic surfactant with the C26H56BrNmolecular structure. It is occasionally used as a disinfectant, synthesis of nanoparticles and organoclays. Neuroblastoma and leukemia cells are more sensitive to it than to any other carcinoma cells.
Didodecyldimethylammonium bromide , Bromide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using CAD.
Methodenparameter
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | AmFm pH 3.0 – 40 mM |
| Flow Rate | 1.0 mL/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |