HPLC Determination of Carvacrol on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carvacrol
Carvacrol (5-Isopropyl-2-methylphenol) is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria. Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | [Model] |
|---|---|
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | 200 nm |