HPLC Determination of Thymol and Carvacrol on Primesep B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol and Carvacrol.
Thymol ( 2-isopropyl-5-methylphenol ) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.
Carvacrol ( 5-Isopropyl-2-methylphenol ), also known as cymophenol, is the main component of the essential oils of the Lamiaceae family of plant. It is used in food and cosmetics for it’s taste and aroma.
Thymol and Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 200 nm |