HPLC Method for Determination of Miconazole in Health Care Products on Primesep B Column
Miconazole is an antifungal medication commonly used to treat fungal infections. Here are some key points about miconazole:
Usage: It’s most often used to treat skin and mucous membrane infections such as vaginal yeast infections, oral thrush, athlete’s foot, jock itch, and ringworm.
Formulations: Miconazole is available in various formulations, including creams, powders, sprays, and suppositories.
Topical Cream: Applied to the skin to treat conditions like athlete’s foot, ringworm, and jock itch. Vaginal Suppositories and Cream: Used for vaginal yeast infections. Oral Gel: Used for oral thrush (a fungal infection in the mouth).
Miconazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 250 nm.
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 30-80%, 20 min |
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |