HPLC Method for Determination of Micafungin on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Micafungin
Micafungin is an anti-fungal medication with the chemical formula C56H71N9O23S . It is used to treat infections including but not limited to candidemia, abscesses, and esophageal candidiasis. It works through inhibiting the production of beta-1,3-glucan. It is only accessible through perscription.
Micafungin can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 270 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Newcrom R1, 2.1 x 100 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 45% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.2 mL/min |
| Detection | UV 270 nm |
| Limit of Detection | 0.02 ppm |