HPLC Method for Determination of Ketoconazole in Nizoral Shampoo on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode
Ketoconazole, also known as Nizoral, is an antifungal compound used to treat dandruff and is commonly available as a shampoo. Ketoconazole can be retained, analyzed, and separated from Benzyl alcohol on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Ammonium formate (AmFm) as the ionic modifier. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 10-50% |
| Buffer | Ammonium Formate pH 3.5-20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |