HPLC Method for Determination of Flunixin on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Flunixin
Flunixin is an analgesic and antipyretic nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C14H11F3N2O2 . It is used primarily by veterinarians in bovine and equine treatments. Prolonged consecutive use can cause health problems due to toxicity. Due to that, it is a prohibited substance under International Federation for Equestrian Sports rules. It is typically produced as a Meglumine salt, which helps stabilize and bulk up the medication in its solid form. You can find detailed UV spectra of Flunixin and information about its various lambda maxima by visiting the following link.
Flunixin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 60% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 252 nm |