HPLC Determination of Vancomycin on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vancomycin.
Vancomycin is a glycopeptide antibiotic with the chemical formula C66H75Cl2N9O24 . It is used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. The WHO classifies it as a critically important human medicine, even including it on the List of Essential Medicines.
Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.
Methodenparameter
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 5-20%, 10 min |
| Buffer | Ammonium Formate pH 3.0 – 10 mM |
| Flow Rate | 1.0 mL/min |
| Detection | 280 nm, CAD |