HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine 5'-diphosphogalactose disodium salt .
Uridine Diphosphate Galactose ( UDP-galactose ) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C15H24N2O17P2 . It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.
UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm – pH 3.0 – 20 mm |
| Flow Rate | 1.0 mL/min |
| Detection | CAD, 262 nm |