HPLC Determination of Succinylcholine on Newcrom AH Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinylcholine
Succinylcholine chloride is a short-acting depolarizing neuromuscular blocker used as a short-term muscle relaxant. Succinylcholine is often used as an adjunct therapy in patients undergoing electroconvulsive shock therapy to control muscle contractions that are induced as a result of the electrical impulses delivered during the procedure.
By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, Succinylcholine can be determinate in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a perchloric acid (HClO4) buffer. UV detection at 200 nm.
Methodenparameter
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 15/85% |
| Buffer | HClO4 – 0.85% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm |