HPLC Determination of Quercetin on Primesep D Column
High Performance Liquid Chromatography (HPLC) Method of Quercetin.
Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, apples, berries, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. It has the chemical formula C15H10O7 .
Quercetin can be retained in HPLC using a Primesep D mixed-mode column using a mobile phase of acetonitrile (ACN), water and Trifluoroacetic acid (TFA) buffer. Detection can be achieved with UV 270 nm , mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).
Methodenparameter
| Column | Primesep D, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 40% |
| Buffer | TFA – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | 270 nm |