HPLC Determination of Propanil on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Propanil .
Propanil is a contact herbicide with the chemical formula C9H9Cl2NO . It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO2 fixation in weeds.
Propanil can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 248 nm |