HPLC Determination of Polyhexanide (PHMB) on PEI Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide PHMB
Polyhexanide (polyhexamethylene biguanide, PHMB) is a polymer used as a disinfectant and antiseptic. We estimated that limit of detection is about 2 ppm for this compounds with this method.
The peak of polyhexanide fractionated on several peaks due to difference in length of the polymer components.
Methodenparameter
| Column | PEI, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 236 nm |