HPLC Determination of Oseltamivir on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oseltamivir .
Oseltamivir belongs to a class of drugs called antivirals, which are used to treat infections caused by viruses. It is used in the treatment of the infection caused by the flu virus (influenza A and influenza B) and may also be used to prevent and treat swine influenza A. It is recommended that it be used within the first 48 hours of the infection’s symptoms. It has the chemical formula C16H28N2O4 .
Oseltamivir can be retained on a Primesep 100 column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulfuric acid (H2SO4) as a buffer. UV Detection 200 nm.
Methodenparameter
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 200 nm |