HPLC Determination of Mycophenolic Acidon Primesep SB Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid .
Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226 nm.
Methodenparameter
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H3PO4- 0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 226 nm |