HPLC Determination of Metalaxyl on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl (UV) .
Metalaxyl , also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate, is a systemic fungicide with the chemical formula C15H21NO4 . It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.
Metalaxyl (UV) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 266 nm |