HPLC Determination of HEPES on Newcrom AH Column
HEPES is a zwitterionic organic chemical buffering agent. It is a very polar compound which is not retained by traditional reverse phase chromatography. HEPES can be separated by mixed-mode hydrophilic interaction chromatography on the Newcrom AH mixed-mode column, which has both hydrophobic and cationic exchange properties. The mobile phase consists of H2O and Methanesulfonic acid. It can be detected using a conductivity detector.
Methodenparameter
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | H2O- 100% |
| Buffer | Methanesulfonic acid – 10 mM |
| Flow Rate | 1.0 mL/min |
| Detection | Conductivity |