HPLC Determination of Glyphosine (bis-PMG) on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosine
Glyphosine is a synthetic plant growth regulator with the chemical formula of C4H11NO8P2 . It is a colorless liquid that works through decreasing chlorophyll production.
Glyphosine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | Formic Acid – 3% |
| Flow Rate | 1.0 mL/min |
| Detection | CAD |