Applikationen / HPLC Determination of Fosfomycin on Primesep SB Column

HPLC Determination of Fosfomycin on Primesep SB Column

HPLC-Applikation • Datum: 2019/06/07
HPLC Determination of Fosfomycin on Primesep SB Column Chromatogramm

Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.

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Methodenparameter

ColumnPrimesep SB
Mobile PhaseMeCN/H2O – 20/80%
BufferAmFm pH 3.0- 20 mM
Flow Rate0.5 mL/min
DetectionCAD (Corona) MS- compatible mobile phase

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