HPLC Determination of Diazepam on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Diazepam .
Diazepam , also known as Valium , is a benzodiazepine. It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system.
Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240 nm.
Methodenparameter
| Column | Newcrom R1, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 30% |
| Buffer | No |
| Flow Rate | 0.2 mL/min |
| Detection | 240 nm |