HPLC Determination of Daptomycin on Primesep 100 column
High Performance Liquid Chromatography (HPLC) Method for Analysis Daptomycin
Daptomycin is a cyclic lipopeptide antibiotic with the chemical formula C72H101N17O26 . It displays a dose-dependent killing activity against a broad range of gram-positive bacteria. It has only minimal activity against anaerobic bacteria and no activity against gram-negative bacteria.
Daptomycin can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |