HPLC Determination of Coenzyme Q10 and Ubiquinol on Primesep D Column

HPLC Method for Coenzyme q10 , Ubiquinol on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Coenzyme q10 , Ubiquinol .

Coenzyme Q10 , also known as ubiquinone , is an enzyme found in animals and is used as a dietary supplement with the chemical formula C59H90O4 . It acts as a redox carrier, which means that it transfers electrons in the mitochondria. The production of ubiquinone depletes with age. Ubiquinol is a reduced form of coenzyme Q10.

The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275 nm.