HPLC Determination of Ciclopirox on Newcrom A Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ciclopirox .
Ciclopirox is a prescription medication with the chemical formula C12H17NO2 . It is used to treat topical fungal infections on skin, nails, and scalp. It is available in a variety of forms from shampoo, to nail lacquer, to cream.
Ciclopirox can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom A, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 45% |
| Buffer | HClO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 300 nm |