HPLC Determination of Benzoyl Peroxide on Newcrom R1 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoyl peroxide.

Hydrogen Peroxide is a chemical compound with the chemical formula H2O 2 . While most assume it to be a colorless liquid, in it’s natural state, it is pale blue. It’s primary uses are found in bleaching, as a disinfectant, and sewage treatment. On occasion, it is used as acne treatment as well. It is used in a variety of production of organic peroxy compounds and inorganic peroxides.

Benzoic Acid is an organic compound with the chemical formula C7H6O2 . It is considered to be the simplest aromatic carboxylic acid. In nature, it can be found in many plants, especially berries. It is used for food preservation of food, as it can inhibit growth of bacteria and mold. While it is safe in food, it can cause irritation to skin, eye, and respiratory systems. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

Benzoyl Peroxide is an organic peroxide with the chemical formula C14H10O4 . It is a commonly used as topical treatment for mild acne because of its antibacterial, anti keratolytic, and comedolytic properties. It is converted to benzoic acid in the skin and primarily targets the pilosebaceous units.

Benzoyl Peroxide can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 235 nm.