HPLC Determination of 6-methyl-5-hepten-2-one on Newcrom R1 Column

HPLC-Applikation • Datum: 2019/05/23
HPLC Determination of 6-methyl-5-hepten-2-one on Newcrom R1 Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Methyl-5-hepten-2-one .

Sulcatone is a ketone used as a mosquito attractant. It is a volatile oil component found in citrus. It can be retained on a Newcrom R1reverse-phase column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. The analytical method is MS-compatible and can also use UV detection at 200 nm.

Methodenparameter

ColumnNewcrom R1
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4 – 0.1%
Flow Rate1.0 mL/min
DetectionUV, 200 nm