HPLC Determination of 6-Benzylaminopurine (N6-Benzyladenine) on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine .
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5 . It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.
6-Benzylaminopurine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate[ buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 272 nm |