HPLC Method for Analysis of 6-Benzylaminopurine (N6-Benzyladenine) on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
6-Benzylaminopurine (N6-Benzyladenine , 6-BAP or BAP) is a synthetic cytokinin commonly used in plant biology and tissue culture to stimulate cell division and growth. It is a potent growth regulator and is applied to enhance plant tissue differentiation, particularly for shoot induction.
6-Benzylaminopurine is one of the most commonly used cytokinins in horticulture and laboratory studies.
6-Benzylaminopurine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 210 nm.
You can find detailed UV spectra of 6-Benzylaminopurine and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 205 nm |
| Sample | 0.1 mg/ml |