HPLC Method for Analysis of 6-Benzylaminopurine on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5 . It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.
6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4-0.2% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 272 nm |