HPLC Method for Analysis of Prochloraz on Newcrom B Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Prochloraz
Prochloraz is an imidazole fungicide with the chemical formula C15H16Cl3N3O2 . It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.
Prochloraz can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |