Alltesta HPLC Method for Separating Corticosteroids such as Prednisone, Prednisolone and Methylprednisolone on Primesep B Column
All three compounds have potent anti-inflammatory effects, making them valuable in treating conditions where inflammation plays a role.
Corticosteroids can be retained, separated, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 245 nm
Methodenparameter
| Column | Primesep B, 3.2 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 35/65% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |