Alltesta HPLC Method for Analysis of Tyramine on Primesep 100
Tyramine is a naturally occurring compound derived from the amino acid tyrosine. It is found in various foods and beverages, especially aged or fermented products such as aged cheeses, cured meats, certain wines, and soy products. Tyramine can affect the release of norepinephrine, a neurotransmitter, and can influence blood pressure.
For people taking monoamine oxidase inhibitors (MAOIs), consuming foods high in tyramine can cause a dangerous spike in blood pressure, known as a hypertensive crisis. This is why individuals on such medications are often advised to follow a low-tyramine diet.
Tyramine can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 275 nm
You can view examples of chromatograms obtained using the Allesta instrument and Sielc columns by clicking here .
Methodenparameter
| Column | Primesep 100, 3.2 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |