Alltesta HPLC Method for Analysis of Linear PEI + (Cu) on PEI Column
Separation type: Liquid Chromatography Reversed-phase
Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 275 nm
Methodenparameter
| Column | PEI, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60%, |
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |