HPLC Method for Analysis of Pantoprazole Sodium in Tablets on Newcrom R1
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole , Pantoprazole sodium
Pantoprazole is a proton pump inhibitor (PPI) with C16H15F2N3O4Smolecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.
Pantoprazole , Pantoprazole sodium can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Triethanolamine buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 4.6 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | TEAPh – 10 mM |
| Flow Rate | 1.0ml/min |
| Detection | UV, 290 nm |