HPLC Determination of Cromolyn Sodium (Sodium cromoglycate) on Newcrom BH Column
High Performance Liquid Chromatography (HPLC) Method for Cromolyn sodium
Cromolyn sodium is a medication used to manage bronchial asthma, allergic rhinitis, and certain allergic eye conditions such as vernal conjunctivitis, keratitis, and keratoconjunctivitis. Cromolyn sodium can be retained in HPLC with a Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and Sulfuric Acid (H2SO4) buffer allowing the use of a UV detector at 240 nm.
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 35/65% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 240 nm |