HPLC Determination of Docusate Sodium in Softgels on Newcrom BH Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Docusate.
Docusate is a laxative and stool softener with the chemical formula C20H37NaO7S . Docusate retains water in the stool, thereby acting as a stool softener to facilitate stool passage. It is found in a variety of forms such as tablets, solution, and syrup over the counter, meaning it can be purchased without a prescription.
Docusate can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | Newcrom BH, 3.2 x 100 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O -75/25% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 200 nm |