HPLC Method for Analysis of Oligonucleotide 5A on Chromni™ Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotide 5 Å.
Oligonucleotide 5 Å is a small RNA molecule with a specific adenine sequence. They are primarily used to inhibit viral and cancer cell growth. In RNA synthesis, they are intermediates in enzymatic synthesis of capped RNAs.
Oligonucleotide 5 Å can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Chromni, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 80-60% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |