HPLC Method for Analysis of Nicotinamide on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide
Nicotinamide , also known as niacinaminde , is a form of vitamin B3 with the chemical formula C6H6N2O2 . It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma.
Nicotinamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |