HPLC Method for Analysis of Neopterin on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Neopterin
Neopterin is an pteridine with the chemical formula C9H11N5O4 . It is indicative of a pro-inflammatory immune status, therefore it typically serves as a marker of the activation of the cellular immune system.
Neopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 5% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |