HPLC Method for Analysis of Naproxen on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Naproxen
Naproxen is a common NSAID used for pain relief and fever reducer. It has the chemical formula C14H14O3 . It works through inhibiting the body’s production of prostaglandins, therefore preventing pain and inflammation. It is available over-the-counter at lower dosages, and through prescription at higher dosages. As a NSAID, it carries a risk of heart attack, stroke, and gastrointestinal bleeding.
Naproxen can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 55% |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |