HPLC Method for Analysis of Methiocarb on Newcrom B Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Methiocarb
Methiocarb is a carbamate pesticide with the chemical formula C11H15NO2S . It works as a acetylcholinesterase inhibitor. It has been in use as an insecticide, bird repellent, acaricide, and molluscicide since 1960s.
Methiocarb can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |