HPLC Method for Analysis of Doxazosin Mesylate in Tablets
High Performance Liquid Chromatography (HPLC) Method for Analysis of Doxazosin mesylate
Doxazosin is a quinazoline derivative with the molecular formula of C23H25N5O5 • CH3SO3H . It is primarily used as a prescription medication for treatment of hypertension and its symptoms. It is an alpha-blocker that relaxes blood vessels and muscles.
Doxazosin mesylate can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 4.6 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | TEAPh – 50 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |