HPLC Method for Analysis of Benztropine mesylate on Primesep SB Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benztropine methylsulfonate .
Benztropine is a medical drug used in treatment of patients with Parkinson’s disease. It works as an acetylcholine blocker decreasing muscle stiffness. Benztropine can be retained in HPLC on Primesep SB mixed-mode column by hydrophobic and anion-exchange properties. The isocratic method uses a mobile phase of acetonitrile (ACN) and water with sulfuric acid buffer and UV detection at 220 nm.
Methodenparameter
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 220 nm |