HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac , Carbamazepine
Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) with the molecular formula C14H11Cl2NO2 . It is a pain killer that also relieves symptoms of arthritis. It is only accessible through a prescription from a medical professional.
Carbamazepine is a anticonvulsant medication with the molecular formula C15H12N2O . It is typically used to treat epilepsy, bipolar disorder, and nerve pain. It works through calming overactive nerves through binding voltage-dependent sodium channels.
Diclofenac , Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 35% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |